Plumbagin attenuates high glucose-induced trophoblast cell apoptosis and insulin resistance via activating AKT/mTOR pathway

Plumbagin, a bioactive phytoconstituent, is isolated from the root of Plumbago zeylanica L. Plumbagin possesses antidiabetic effect to mediate glucose homeostasis, wound healing and diabetic nephropathy. However, involvement plumbagin in gestational diabetes mellitus (GDM) has not been reported yet. Trophoblast cell line (HTR8/SVneo) was incubated with high establish model GDM. Cell viability proliferation were detected by MTT EdU staining. Flow cytometry used investigate apoptosis. HTR8/SVneo reduced or incubation plumbagin. restored induced glucose. attenuated glucose-induced apoptosis cells through upregulation Bcl-2 down-regulation Bax, cleaved caspase-3 caspase-9. Protein expression transporter type 4 (GLUT-4), insulin receptor (INSR)-B INSR substrate (IRS1) decreased but increased The suppressive effects on phosphorylation AKT mTOR reversed improved resistance activation AKT/mTOR pathway, suggesting that might be as potential strategy for prevention